Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin
Figure 4
Protein expression of NOX1, NOXA1, NOXO1, and p22PHOX in the colon of TNFα-treated mice: effect of apocynin. (a) Mice were treated with TNFα (10 μg · kg−1, ip) in the presence or absence of apocynin (25 mg · kg−1, ip) as described in Figure 1(a) (C in the figure is for control untreated mice). Proteins were extracted and 50 μg was subjected to SDS-PAGE and Western blot as described in Section 2 using NOX1, NOXA1, NOXO1, or p22PHOX specific antibodies. Mice -actin served as a control for protein loading and transfer; (b) densitometric analysis of the ratio of NOX1, NOXA1, NOXO1, or p22PHOX protein expression to the total amount of -actin. Data are expressed as means ± S.E.M from mice in each group. , , and versus control; , , and versus TNFα. (c) Upper: Western blot analysis of DUOX2 expression using specific antibodies. Mice -actin served as a control for protein loading and transfer. Lower: densitometric analysis of the ratio of DUOX2 protein expression to the total amount of -actin. Data are expressed as means ± S.E.M from mice in each group. versus control; versus TNFα.
