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Mediators of Inflammation
Volume 2014, Article ID 349476, 14 pages
Research Article

Can the TLR-4-Mediated Signaling Pathway Be “A Key Inflammatory Promoter for Sporadic TAA”?

1Unit of Cardiac Surgery, Department of Surgery and Oncology, University of Palermo, 90127 Palermo, Italy
2Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy
3Department of Pathologic Anatomy, University of Palermo, 90127 Palermo, Italy

Received 19 December 2013; Accepted 18 June 2014; Published 10 July 2014

Academic Editor: Massimiliano M. Corsi Romanelli

Copyright © 2014 Giovanni Ruvolo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In this section, we reported in detail the procedure used for evaluating aortic transverse diameter sizes. In addition, criteria, definitions and grading systems for tissue sample collection, staining, histopathological and immunohistochemical assessment, ELISA for quantifying inflammatory plasma molecules, Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) testing for evaluating apoptosis, semiquantitative MMP-9 evaluation, assessment of mean terminal restriction fragment length and genotyping were also described in detail. Furthermore, we reported in Table 1S information about the 6 genes and 10 SNPs analysed and their Biological Effects. In Figures 1S and 2S we reported the control aortas and histo-pathological abnormalities in aorta tissues of S-TAA patients and medial apoptosis and MMP-9 amounts in tissue samples, reciprocally.

  1. Supplementary Material