Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications
Table 2
Immunohistochemical evaluation grading score for CD4/CD8, CD20, and CD68 cells and FGF-β, COX2, and TGF-β of each specimen. (a) Expression of the examined markers in the control group, (b) expression of the markers in the group of mild Graves’ orbitopathy, and (c) expression of the markers in the group of severe Graves’ orbitopathy.
(a)
Number
Classification
CD4/CD8
CD20
CD68
COX-2
FGF
TGF
1
Contrl.
0-0
0
1
0
0
0
2
Contrl.
0-0
0
0
0
0
0
3
Contrl.
0-0
0
0
0
0
0
4
Contrl.
0-0
0
0
0
0
0
5
Contrl
0-0
0
0
0
0
0
6
Contrl
0-0
0
1
0
1
0
7
Contrl.
0-1
1
1
0
1
0
(b)
Number
Classification
CAS score
CD4/CD8
CD20
CD68
COX-2
FGF
TGF
1
Mild GO
1
0-0
0
1
0
1
1
2
Mild GO
3
1-0
0
2
0
2
1
3
Mild GO
2
1-0
0
1
0
1
1
4
Mild GO
3
1-0
0
2
0
2
1
5
Mild GO
1
1-0
0
1
0
1
1
6
Mild GO
3
1-1
0
2
0
1
1
7
Mild GO
1
1-1
0
1
0
1
1
8
Mild GO
3
0-0
0
2
0
1
2
(c)
Number
Classification
CAS score
CD4/CD8
CD20
CD68
COX-2
FGF
TGF
1
Severe GO
10
1-0
0
2
0
2
2
2
Severe GO
10
1-0
0
2
0
2
2
3
Severe GO
9
1-0
0
2
0
2
1
4
Severe GO
8
1-0
0
2
0
2
2
5
Severe GO
7
1-0
0
1
0
1
1
6
Severe GO
10
1-0
0
2
0
2
2
7
Severe GO
10
1-0
0
2
0
2
2
8
Severe GO
9
1-0
2
2
0
2
2
9
Severe GO
10
1-1
0
2
0
2
2
10
Severe GO
10
2-1
0
2
0
2
2
11
Severe GO
5
1-0
0
1
0
1
1
12
Severe GO
10
1-0
0
2
0
2
2
13
Severe GO
10
1-0
0
2
0
2
2
14
Severe GO
6
1-0
0
2
0
1
1
15
Severe GO
5
0-0
0
1
0
1
1
16
Severe GO
5
0-0
0
1
0
1
1
17
Severe GO
5
1-0
0
1
0
1
1
18
Severe GO
8
1-0
0
1
0
2
1
19
Severe GO
10
1-0
0
2
0
2
2
20
Severe GO
7
1-0
0
2
0
1
2
21
Severe GO
5
1-0
0
1
0
2
2
22
Severe GO
10
1-0
0
2
0
2
2
Legend for Tables 2(a), 2(b), and 2(c): immunohistochemistry score: 0-less than 10% positive in 10 representative high power fields (HPF). 1-10%–50% positive cells in 10 HPF. 2-more than 50% positive cells in 10 HPF. Immunohistochemistry was done using DAB chromogen (brown staining). Ctrl: controls.