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Mediators of Inflammation
Volume 2014, Article ID 573825, 7 pages
http://dx.doi.org/10.1155/2014/573825
Research Article

IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis

1Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil
2Laboratory of Biomedical Sciences, Federal University of Uberlândia, Rua 20, No. 1600, 38304-402 Ituiutaba, MG, Brazil
3Course of Tropical Medicine and Infectology, Laboratory of Parasitology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil
4Laboratory of Histology and Embryology, Federal University of Uberlândia, Avenida Pará, No. 1720, 38400-902 Uberlândia, MG, Brazil
5Laboratory of Immunology, Federal University of Uberlândia, Avenida Pará, No. 1720, 38400-902 Uberlândia, MG, Brazil

Received 25 April 2014; Revised 11 July 2014; Accepted 12 July 2014; Published 17 August 2014

Academic Editor: Christophe Chevillard

Copyright © 2014 Jéssica Líver Alves Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to measure the synthesis of Th1 and Th2 cytokines by mononuclear cells after culture with live T. gondii and identified Th17 (CD4+) and Tc17 (CD8+) cells in toxoplasma-seronegative and toxoplasma-seropositive parturient and nonpregnant women. Cytometric bead arrays were used to measure cytokine levels (IL-2, TNF-α, IFN-γ, IL-4, IL-5, and IL-10); immunophenotyping was used to characterize Th17 and Tc17 cells, and the cells were stained with antibodies against CD4+ and CD8+ T cells expressing IL-17. The addition of tachyzoites to cell cultures induced the synthesis of IL-5, IL-10, and TNF-α by cells from seronegative parturient women and of IL-5 and IL-10 by cells from seropositive, nonpregnant women. We observed a lower level of IL-17-expressing CD4+ and CD8+ T lymphocytes in cultures of cells from seronegative and seropositive parturient and nonpregnant women that were stimulated with tachyzoites, whereas analysis of the CD4+ and CD8+ T cell populations showed a higher level of CD4+ T cells compared with CD8+ T cells. These results suggest that the cytokine pattern and IL-17-expressing CD4+ and CD8+ T lymphocytes may have important roles in the inflammatory response to T. gondii, thus contributing to the maintenance of pregnancy and control of parasite invasion and replication.