Anti-Inflammatory Effects of IKK Inhibitor XII, Thymulin, and Fat-Soluble Antioxidants in LPS-Treated Mice
The effects of IKK Inhibitor XII and antioxidant-rich diet on phosphorylation of RelA, IKK, and SAPK/JNK and on the expression of TLR4 and heat shock proteins in the splenic lymphocytes from inflammation-bearing mice. The animal’s groups that was indicated in Figure 1 were used (1, control; 2, IB mice; 3, IB + Inh5; 4, IB + Inh10; 5, IB + Inh20; 6, IB + diet; 7, IB + diet + Inh20). Western blot analysis of extracts from isolated mice lymphocytes was provided using corresponding antibodies or anti-GAPDH antibody (bottom). Blot pictures show a single representative experiment from four independent experiments. Histograms below protein bands show protein levels calculated as mean relative units correspondingly to internal control and are the results of blots densitometry by program QAPA from four independent experiments. Significantly different from control, . ∧Significantly different from LPS-group, .