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Mediators of Inflammation
Volume 2014, Article ID 728939, 6 pages
http://dx.doi.org/10.1155/2014/728939
Research Article

Circumsporozoite Protein-Specific -Restricted CD8+ T Cells Mediate Protective Antimalaria Immunity in Sporozoite-Immunized MHC-I- Transgenic Mice

1HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, 455 First Avenue, New York, NY 10016, USA
2Department of Pathology, New York University School of Medicine, 545 First Avenue, New York, NY 10016, USA

Received 24 April 2014; Accepted 23 June 2014; Published 15 July 2014

Academic Editor: Mauricio Martins Rodrigues

Copyright © 2014 Jing Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Although the roles of CD8+ T cells and a major preerythrocytic antigen, the circumsporozoite (CS) protein, in contributing protective antimalaria immunity induced by radiation-attenuated sporozoites, have been shown by a number of studies, the extent to which these players contribute to antimalaria immunity is still unknown. To address this question, we have generated C57BL/6 (B6) transgenic (Tg) mice, expressing molecules under the MHC-I promoter, called MHC-I- -Tg mice. In this study, we first determined that a single immunizing dose of IrPySpz induced a significant level of antimalaria protective immunity in MHC-I- -Tg mice but not in B6 mice. Then, by depleting various T-cell subsets in vivo, we determined that CD8+ T cells are the main mediator of the protective immunity induced by IrPySpz. Furthermore, when we immunized (MHC-I- -Tg × CS-Tg) F1 mice with IrPySpz after crossing MHC-I- -Tg mice with PyCS-transgenic mice (CS-Tg), which are unable to mount PyCS-specific immunity, we found that IrPySpz immunization failed to induce protective antimalaria immunity in (MHC-I- -Tg × CS-Tg) F1 mice, thus indicating the absence of PyCS antigen-dependent immunity in these mice. These results indicate that protective antimalaria immunity induced by IrPySpz in MHC-I- -Tg mice is mediated by CS protein-specific, -restricted CD8+ T cells.