Neutrophil Cathepsin G, but Not Elastase, Induces Aggregation of MCF-7 Mammary Carcinoma Cells by a Protease Activity-Dependent Cell-Oriented Mechanism
Figure 3
Induction of MCF-7 cell aggregation by serine protease treatment of FBS- or FN-coated wells. FBS-coated wells (a) or FN-coated wells (b) were treated overnight with cathepsin G (), elastase (●), chymotrypsin (), or trypsin (). Next, MCF-7 cells suspended in 1% BSA-medium were seeded and incubated for 24 h. The cell aggregation index was evaluated as described in the legend of Figure 2. The results are expressed as means (). When the bars are not shown, they are smaller than the size of the symbols. Lower photos: images of MCF-7 cells at 24 h after cultivation in protease-treated FBS-coated wells. Original magnification: ×100.
