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Mediators of Inflammation
Volume 2014, Article ID 971409, 12 pages
http://dx.doi.org/10.1155/2014/971409
Research Article

Neutrophil Cathepsin G, but Not Elastase, Induces Aggregation of MCF-7 Mammary Carcinoma Cells by a Protease Activity-Dependent Cell-Oriented Mechanism

Laboratory of Host Defense, Department of Pharma-Sciences, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan

Received 3 September 2013; Revised 20 December 2013; Accepted 7 February 2014; Published 2 April 2014

Academic Editor: Simi Ali

Copyright © 2014 Satoru Yui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1: Elastase does not increase MCF-7 cell motility.

Supplementary figure 2: The enzymatic activity of cathepsin G and Elastase bound to the bottoms of FN-coated plates.

Supplementary figure 3: Effect of PMSF treatment against protease-treated FPS-coated wells on the induction of MCF-7 cell aggregation.

Supplementary figure 4: PLL-FITC was not a substrate for cathepsin G and Elastase.

  1. Supplementary Figures