LPS from P. gingivalis and Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis

Figure 5

Catalase (CAT) occurrence in healthy and inflamed human tissue. Healthy gingival epithelium (a), periodontal ligament (b), and samples of gingivitis (c) or periodontitis (d) were obtained after the approval of the Ethics Committee of the University of Bonn and parental as well as patient’s allowance (). Polyclonal primary antibody against catalase (Abcam, Cambridge, UK) was used in a concentration of 1 : 100. No immunostaining could be found in the healthy gingival epithelium. In the healthy PDL, predominantly PDL cells located near to the tooth root surfaces were stained (Figure 5(b), red arrow). In gingivitis tissue, a weak staining of gingival keratinocytes similar to healthy tissue could be seen (Figure 5(c)). Instead, in the tissue samples of patients with periodontitis a strong immunoreactivity was observed in the subepithelial layer probably referring to nuclei staining of immune cells like leukocytes. Red arrows indicate examples for the typical localisation (Figure 5(d)). The scale bars indicate 200 μm in the surveys and 50 μm in the higher magnifications, respectively.

(a)

(b)

(c)

(d)