Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy

Table 2

Immunohistochemical evaluation grading score for TCR and CD3 of each specimen.

Number	Group	TCR	CD3
1	Control	0	0
2	Control	0	0
3	Control	0	0
4	Control	0	0
5	Control	0	0
6	Control	0	0
7	Control	0	0
8	Mild GO	0	0
9	Mild GO	1	1
10	Mild GO	1	1
11	Mild GO	1	1
12	Mild GO	1	1
13	Mild GO	0	0
14	Severe GO	1	1
15	Severe GO	1	1
16	Severe GO	1	1
17	Severe GO	1	1
18	Severe GO	2	1
19	Severe GO	1	1
20	Severe GO	1	1
21	Severe GO	2	1
22	Severe GO	2	1
23	Severe GO	2	2
24	Severe GO	1	1
25	Severe GO	2	1
26	Severe GO	2	1
27	Severe GO	1	1
28	Severe GO	1	1
29	Severe GO	1	1
30	Severe GO	1	1
31	Severe GO	1	1
32	Severe GO	2	1
33	Severe GO	1	1

Legend for Table 2: Immunohistochemistry scores are as follows:
0: less than 10% positive in 10 representative high power fields (HPF),
1: 11%–50% positive cells in 10 HPF,
2: more than 50% positive cells in 10 HPF.
Immunohistochemistry was done using DAB chromogen (brown staining).