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Mediators of Inflammation
Volume 2015, Article ID 352356, 11 pages
http://dx.doi.org/10.1155/2015/352356
Research Article

Plasma Nitration of High-Density and Low-Density Lipoproteins in Chronic Kidney Disease Patients Receiving Kidney Transplants

1Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY, USA
2The Department of Veterans Affairs New York Harbor Healthcare System, Brooklyn, NY, USA
3Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY, USA
4Department of Surgery, SUNY Downstate Medical Center, Brooklyn, NY, USA

Received 19 May 2015; Revised 14 October 2015; Accepted 19 October 2015

Academic Editor: Aaron L. Sverdlov

Copyright © 2015 Ahmed Bakillah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Functional abnormalities of high-density lipoprotein (HDL) could contribute to cardiovascular disease in chronic kidney disease patients. We measured a validated marker of HDL dysfunction, nitrated apolipoprotein A-I, in kidney transplant recipients to test the hypothesis that a functioning kidney transplant reduces serum nitrated apoA-I concentrations. Methods. Concentrations of nitrated apoA-I and apoB were measured using indirect sandwich ELISA assays on sera collected from each transplant subject before transplantation and at 1, 3, and 12 months after transplantation. Patients were excluded if they have history of diabetes, treatment with lipid-lowering medications or HIV protease inhibitors, prednisone dose > 15 mg/day, nephrotic range proteinuria, serum creatinine > 1.5 mg/dL, or active inflammatory disease. Sera from 18 transplanted patients were analyzed. Four subjects were excluded due to insufficient data. Twelve and eight patients had creatinine < 1.5 mg/dL at 3 and 12 months after transplantation, respectively. Results. Nitrated apoA-I was significantly reduced at 12 months after transplantation (). The decrease in apoA-I nitration was associated with significant reduction in myeloperoxidase (MPO) activity (). In contrast to apoA-I, nitrated apoB was not affected after kidney transplantation. Conclusions. Patients with well-functioning grafts had significant reduction in nitrated apoA-I 12 months after kidney transplantation. Further studies are needed in a large cohort to determine if nitrated apoA-I can be used as a valuable marker for cardiovascular risk stratification in chronic kidney disease.