CXCR6 Expression Is Important for Retention and Circulation of ILC Precursors

Figure 5

Normal intestinal ILC compartments in CXCR6-deficient mice are compensated by higher proliferative capacities of in situ progenitor cell. (a) Percentages of ILC subsets in Lin⁻ (as defined in Figure 3(b)) in adult LP from Rag2^−/−  Cxcr6^+/+ (wt, blue losange), Cxcr6^Gfp/+ (HZ, red square), or Cxcr6^Gfp/Gfp (KO, green triangle) adult mice. (b) Histograms depicting levels of CD62L, CD69, CD44, CXCR6-GFP, and α₄β₇ in Lin⁻ (filled gray) or ILC subsets (as defined in Figure 3(b)) in adult LP (upper panels) or mesenteric lymph nodes (mLN, lower panels) from Rag2^−/−  Cxcr6^Gfp/Gfp mice. (c) Histograms of total LP Lin⁻ cells (filled gray) or ILC2 cells (red line) for Ki67 expression and DAPI levels. ((d), (e)) Percentages of Ki67⁺ cells (d) and DAPI⁺ cells (e) among ILC subsets (as defined in Figure 4(b)) in LP from Rag2^−/−  Cxcr6^+/+ (wt, blue losange), Cxcr6^Gfp/+ (HZ, red square), or Cxcr6^Gfp/Gfp (KO, green triangle) adult mice. (f) Flow cytometry of LP in situ ILCP (upper panel) and CXCR6-GFP and ID2-YFP expression of LP in situ ILCP (lower panels) from Cxcr6^Gfp/+  Id2^Yfp/+ mice. (g) Percentages of IL-7Rα⁺ precursors in Lin⁻ (left), Ki67⁺ (middle), and DAPI⁺ (right) cells among IL-7Rα⁺ precursors in LP from Rag2^−/− Cxcr6^+/+ (wt, blue losange), Cxcr6^Gfp/+ (HZ, red square), or Cxcr6^Gfp/Gfp (KO, green triangle) adult mice. Results are from 3 pooled experiments ((a), (c), (d): wt: , HZ: , KO: ) or from 2 pooled experiments ((e), (f), GF, wt: , HZ: , KO: ) or are representative of 3 experiments (b). In ((a), (d), (e), (g)), each dot represents a single mouse. Statistical data are displayed with mean and SEM (Student’s unpaired bilateral test, ; ).

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