Table of Contents Author Guidelines Submit a Manuscript
Mediators of Inflammation
Volume 2015 (2015), Article ID 484357, 10 pages
http://dx.doi.org/10.1155/2015/484357
Research Article

Cardiac-Restricted IGF-1Ea Overexpression Reduces the Early Accumulation of Inflammatory Myeloid Cells and Mediates Expression of Extracellular Matrix Remodelling Genes after Myocardial Infarction

1National Heart and Lung Institute, Imperial College London, London, UK
2Australian Regenerative Medicine Institute, EMBL Australia, Monash University, Clayton, Melbourne, VIC, Australia
3The Jackson Laboratory, Bar Harbor, ME, USA

Received 23 July 2015; Accepted 13 September 2015

Academic Editor: Michael Schnoor

Copyright © 2015 Enrique Gallego-Colon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary 1. IGF-1Ea relative levels in the heart after myocardial infarct.

Supplementary 2. Double CD206+/Ly6C+ population.

Supplementary 3. Gating strategy for infiltrating immune cells after myocardial infarct.

Supplementary 4. Fluorescence minus one (FMO) controls plots for Ly6G and CD11c. Based on the gating strategy in Supplementary figure 3, FMO controls were used to set the threshold gate. Single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with anti-CD11b, -CD45, –Ly-6G, –Ly-6C, -F4/80, -CD206 -CD11c except for the fluorochrome being negatively gated. For the Ly6G FMO control, single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with all the fluorochromes except Ly6G. For the CD11c FMO controls single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with all the fluorochromes except CD11c.

Supplementary 5. Fluorescence minus one (FMO) controls plots for CD11b and F4/80. Based on the gating strategy in Supplementary figure 3, FMO controls were used to set the threshold gate. Single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with anti-CD11b, -CD45, –Ly-6G, –Ly-6C, -F4/80, -CD206 -CD11c except for the fluorochrome being negatively gated. For the CD11b FMO control, single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with all the fluorochromes except CD11b. For the F4/80 FMO controls single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with all the fluorochromes except F4/80.

Supplementary 6. Fluorescence minus one (FMO) controls plots for Ly6C and CD206. Based on the gating strategy in Supplementary figure 3, FMO controls were used to set the threshold gate. Single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with anti-CD11b, -CD45, –Ly-6G, –Ly-6C, -F4/80, -CD206 -CD11c except for the fluorochrome being negatively gated. For the Ly6C FMO control, single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with all the fluorochromes except Ly6C. For the CD206 FMO controls single cell suspensions isolated from hearts of mice uninjured and post-MI were stained with all the fluorochromes except CD206.

Supplementary table 1. Echocardiographic measurements and analysis performed at 1, 3, 5, 7 and 28 days after MI.

  1. Supplementary Material