Sphingosine-1-Phosphate/Sphingosine-1-Phosphate Receptor 2 Axis Can Promote Mouse and Human Primary Mast Cell Angiogenic Potential through Upregulation of Vascular Endothelial Growth Factor-A and Matrix Metalloproteinase-2

Figure 2

Human MC secrete proangiogenic factors upon exposure to S1P in a S1PR2-dependent manner. (a) VEGF-A levels were measured in the supernatants of activated human MC in the absence (open bars, vehicle (DMSO/PBS/4 mg/mL fatty acid-free BSA)) or presence of a 30-minute pretreatment with CYM-5442 (filled bars, S1PR1 agonist, 1 μM) or with JTE-013 (gray bars, S1PR2 antagonist, 1 μM), followed by the indicated stimuli similar to Figure 1(b). (b) Matrix metalloproteinase- (MMP-) 2 levels were measured in supernatants of MC activated exactly as described in Figure 1(b). All supernatants were collected 24 hours after stimulation. Activation experiments were conducted using five independent human skin MC populations generated from five donors. Activation was conducted in triplicate determinations and measurements were conducted in duplicate determinations for each individual determination. When reaching significance, statistics are indicated in each figure. Error bars show standard error of means. .

(a)

(b)