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Mediators of Inflammation
Volume 2016, Article ID 2979846, 9 pages
Research Article

Interleukin-17A Gene Variability in Patients with Type 1 Diabetes Mellitus and Chronic Periodontitis: Its Correlation with IL-17 Levels and the Occurrence of Periodontopathic Bacteria

1Clinic of Stomatology, Institutions Shared with St. Anne’s Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic
2Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic
3Institute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, 128 08 Prague, Czech Republic

Received 25 September 2015; Revised 30 December 2015; Accepted 3 January 2016

Academic Editor: Kong Chen

Copyright © 2016 Petra Borilova Linhartova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Interleukin-17 contributes to the pathogenesis of type 1 diabetes mellitus (T1DM) and chronic periodontitis (CP). We analyzed IL-17A −197A/G and IL-17F +7488C/T polymorphisms in T1DM and CP and determined their associations with IL-17 production and occurrence of periopathogens. Totally 154 controls, 125 T1DM, and 244 CP patients were genotyped using 5′ nuclease assays. Bacterial colonization was investigated by a DNA-microarray kit. Production of IL-17 after in vitro stimulation of mononuclear cells by mitogens and bacteria was examined by the Luminex system. Although no differences in the allele/genotype frequencies between patients with CP and T1DM + CP were found, the IL-17A −197 A allele increased the risk of T1DM (). Levels of HbA1c were significantly elevated in carriers of the A allele in T1DM patients (). Production of IL-17 by mononuclear cells of CP patients (unstimulated/stimulated by Porphyromonas gingivalis) was associated with IL-17A A allele (). IL-17A polymorphism increased the number of Tannerella forsythia and Treponema denticola in patients with CP and T1DM + CP, respectively (). IL-17A gene variability may influence control of T1DM and the “red complex” bacteria occurrence in patients with CP and T1DM + CP. Our findings demonstrated the functional relevance of the IL-17A polymorphism with higher IL-17 secretion in individuals with A allele.