TLR Signalling Pathways Diverge in Their Ability to Induce PGE₂

Figure 3

TLRs differentially activate the MAPK and NF-κB pathways. DCs were stimulated with TLR agonists as indicated in Figure 1 for 30 min. After cell lysis, extracts were blotted against phospho-p38, phospho-ERK1/2, and phospho-MSK1. Nuclear extracts were blotted against NF-κB p65. β-actin and Lamin B represent loading controls for total and nuclear proteins, respectively. The image depicts results obtained in one representative donor out of three.