Anti-Inflammatory Effects of Hyperbaric Oxygenation during DSS-Induced Colitis in BALB/c Mice Include Changes in Gene Expression of HIF-1α, Proinflammatory Cytokines, and Antioxidative Enzymes

Figure 3

HBO₂ induced changes in CD4⁺ and CD8⁺ T cell frequencies in the colon of BALB/c mice with DSS induced colitis. BALB/c mice at the age of 10–12 weeks were randomly assigned into 4 groups ( min. 4 mice/group/experiment): CTRL: control mice, CTRL + HBO₂: control mice undergoing HBO₂ (60 min/2.4 ATM, 2x/day, days 1–8), DSS: mice receiving dextran sodium sulphate (DSS, 5% w/v, days 1–7), and DSS + HBO₂: DSS treated mice undergoing HBO₂. (a) Representative dot plots of colon lamina propria lymphocyte obtained by flow cytometry, illustrating the gating strategy for CD4⁺ and CD8⁺ lymphocytes, and (b) measured frequencies of colon CD4⁺ and CD8⁺ lymphocytes. Doublets were excluded by forward scatter area (FSC-A) versus forward scatter height (FSC-H) and the dead cells using 7-AAD. Data are presented as mean s.e.m.% of single, live lymphocytes. ^∗Statistically different from CTRL, ; statistically different from CTRL + HBO₂.

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