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Mediators of Inflammation
Volume 2016 (2016), Article ID 7141430, 19 pages
Research Article

Anti-Inflammatory Effects of Hyperbaric Oxygenation during DSS-Induced Colitis in BALB/c Mice Include Changes in Gene Expression of HIF-1α, Proinflammatory Cytokines, and Antioxidative Enzymes

1Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia
2Department of Biology, University of Osijek, 31 000 Osijek, Croatia
3Department of Immunology and Biotechnology, Faculty of Medicine, University of Pécs, 7624 Pécs, Hungary
4Lymphoid Organogenesis Research Group, Szentágothai Research Center, University of Pécs, 7624 Pécs, Hungary
5Department of Histology and Embryology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia

Received 25 March 2016; Revised 8 July 2016; Accepted 12 July 2016

Academic Editor: Fábio Santos Lira

Copyright © 2016 Sanja Novak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Relative mRNA expression of VEGF (A), and PGK1 gene (B) in colon and their correlation to the HIF-1α gene expression. Relative mRNA expression was determined by real-time PCR, and the measured genes were normalized to the HPRT1 gene expression. BALB/c mice at the age of 10–12 weeks were randomly assigned into 4 groups (n = 5/group/experiments) CTRL—control mice, CTRL+HBO2—control mice undergoing HBO2 (60 min/2.4 ATM, 2x/day, days 1–8), DSS—mice receiving dextran sodium sulphate (DSS, 5% w/v, days 1–7), and DSS+HBO2—DSS treated mice undergoing HBO2. Data are presented as mean ± s.e.m. of two independent experiments, each with min. 5 mice/group. *statistically different from CTRL, P < 0.05; statistically different from CTRL+HBO2, P < 0.05.

  1. Supplementary Material