Anti-Inflammatory Effects of Hyperbaric Oxygenation during DSS-Induced Colitis in BALB/c Mice Include Changes in Gene Expression of HIF-1α, Proinflammatory Cytokines, and Antioxidative Enzymes

Table 1

Primer sequences, PCR product length, and primers annealing temperature used for qPCR analysis.

Gene		Sequence	PCR product length/bp	Annealing temperature/°C
HIF-1α	For	TGACGGCGACATGGTTTACA	280	63
	Rev	AATATGGCCCGTGCAGTGAA
SOD1	For	GGAAGCATGGCGATGAAAGC	80	56
	Rev	GCCTTCTGCTCGAAGTGGAT
GPx1	For	TCCAGTATGTGTGCTGCTCG	249	63
	Rev	GTGTCCGAACTGATTGCACG
CAT	For	GGTGCCCCCAACTATTACCC	141	61
	Rev	GAATGTCCGCACCTGAGTGA
IL-1β	For	GCCTTGGGCCTCAAAGGAAAGAATC	282	66
	Rev	GGAAGACACAGATTCCATGGTGAAG
IL-2	For	CTCTGCGGCATGTTCTGGAT	163	65
	Rev	AGAAAGTCCACCACAGTTGCT
IL-6	For	GCTGGAGTCACAGAAGGAGTGGC	117	63
	Rev	GGCATAACGCACTAGGTTTGCCG
HPRT1	For	TCAGTCAACGGGGGACATAAA	142	59
	Rev	GGGGCTGTACTGCTTAACCAG