Research Article

Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing

Figure 1

AKT functional analyses. The analyses of pAKT and total AKT (by arbitrary units A.U.) were revealed through confocal immunofluorescence in BV-2 cells. (a) IF of treated cells. (A) Controls. (B) LPS. (C) Wortmannin. (D) LY294002. (E) Wortmannin+LPS. (F) LY294002+LPS. (G) TVE+LPS. Graphs of fluorescence intensity and pAKT modulation according to the IF spectra reported in Figure 1. (b) Dose-effect evaluation of pAKT and total AKT expressions. (c) Uptake evaluation by spectrophotometric analyses. (A) OD of 7 TVE concentrations: %, %, %, %, , %, and %. (B) Bar graph reporting the difference of TVE concentrations in the medium at the beginning (T0) and the end (T1) of experiments.
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