Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury

Figure 5

P5 reduced alveolar leukocyte recruitment. (a, b) Saline (Sham) or P5 (8 mg/kg, P5) was injected twice though ip at 2 and 16 hr post-ALI induction in mice. At 4 hr after second administration, mouse lungs were fixed in situ with PF, cut into 10 μm sections, and stained with H&E. The counts of alveoli with and without leukocytes were obtained in a double-blind manner. , . (c) Mouse BALF samples were obtained as described in Figure 3(b). Flow cytometry analysis of BALF was performed at Body Fluid mode to obtain total counts of alveolar leukocytes. , . (d) After centrifugation at 1,000 g, cell pellets from BALF were resuspended in serum and subjected to Wright-Giemsa staining on a glass slide. The counts of monocyte/macrophage (MN), neutrophil (PMN), and lymphocyte (Lym) and the ratio (%) of each cell type were obtained. .

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