Mediators of Inflammation / 2020 / Article / Tab 5

Research Article

Plasma and Cellular Forms of Fibronectin as Prognostic Markers in Sepsis

Table 5

The appearance of FN monomers, FN dimers, and FN-fibrin complexes in the plasma of patients with sepsis determined on the basis of immunoblotting after SDS-AGE patterns.

SurvivorsNonsurvivorsControl value

FN monomerOccurrence (%08080880.745
Relative amount (median (IQR))4.4 (3.5-6.7)5.6 (3.8-7.0)5.7 (3.8-6.6)0.9401
1.0002
1.0003
FN dimerOccurrence (%)100100100
Relative amount (median (IQR))63.0 (52.5-73.8)56.8 (46.8-64.4)92.7 (92.4-95.9)0.3421
<0.0012
<0.0031
FN-fibrin complexes
 IOccurrence (%)10010018<0.001
Relative amount (median (IQR))28.5 (16.0-31.8)29.0 (23.5-32.7)3.8 (3.7-8.7)0.7661
0.0302
0.0073
 IIOccurrence (%)89946<0.001
Relative amount (median (IQR))5.3 (2.5-11.3)7.4 (3.8-12.9)1.8 (1.8-1.9)0.6561
0.5542
0.3103
 IIIOccurrence (%)46670<0.001
Relative amount (median (IQR))3.2 (1.8-6.0)3.0 (1.6-6.3)Not detected1.000
 IVOccurrence (%)203300.021
Relative amount (median (IQR))1.1 (0.7-1.7)1.3 (0.7-2.0)Not detected1.000

value represents difference between subgroups: 1—survivors vs. nonsurvivors; 2—survivors vs. control; 3—nonsurvivors vs. control. The comparison of continuous variables between two independent groups (nonsurvivors vs. survivors) was performed using the Mann-Whitney test. The comparison of continuous variables across three groups (nonsurvivors, survivors, and control) was performed using the Kruskal-Wallis test, and a post hoc was used to compare subgroups. Plasma FN forms were revealed by SDS-agarose immunoblotting (see Figure 3(b)). Occurrence is the ratio of the number of samples containing the FN form to the total number of samples. The relative amount of the FN band is the percentage of the total number of pixels found in the electrophoresis path.

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