Plasma and Cellular Forms of Fibronectin as Prognostic Markers in Sepsis

Table 5

The appearance of FN monomers, FN dimers, and FN-fibrin complexes in the plasma of patients with sepsis determined on the basis of immunoblotting after SDS-AGE patterns.

		Survivors	Nonsurvivors	Control	value
FN monomer	Occurrence (%0	80	80	88	0.745
	Relative amount (median (IQR))	4.4 (3.5-6.7)	5.6 (3.8-7.0)	5.7 (3.8-6.6)	0.940^1
					1.000^2
					1.000^3
FN dimer	Occurrence (%)	100	100	100	—
	Relative amount (median (IQR))	63.0 (52.5-73.8)	56.8 (46.8-64.4)	92.7 (92.4-95.9)	0.342^1
					<0.001^2
					<0.00^31
FN-fibrin complexes
I	Occurrence (%)	100	100	18	<0.001
	Relative amount (median (IQR))	28.5 (16.0-31.8)	29.0 (23.5-32.7)	3.8 (3.7-8.7)	0.766^1
					0.030^2
					0.007^3
II	Occurrence (%)	89	94	6	<0.001
	Relative amount (median (IQR))	5.3 (2.5-11.3)	7.4 (3.8-12.9)	1.8 (1.8-1.9)	0.656^1
					0.554^2
					0.310^3
III	Occurrence (%)	46	67	0	<0.001
	Relative amount (median (IQR))	3.2 (1.8-6.0)	3.0 (1.6-6.3)	Not detected	1.000
IV	Occurrence (%)	20	33	0	0.021
	Relative amount (median (IQR))	1.1 (0.7-1.7)	1.3 (0.7-2.0)	Not detected	1.000

value represents difference between subgroups: ¹—survivors vs. nonsurvivors; ²—survivors vs. control; ³—nonsurvivors vs. control. The comparison of continuous variables between two independent groups (nonsurvivors vs. survivors) was performed using the Mann-Whitney test. The comparison of continuous variables across three groups (nonsurvivors, survivors, and control) was performed using the Kruskal-Wallis test, and a post hoc was used to compare subgroups. Plasma FN forms were revealed by SDS-agarose immunoblotting (see Figure 3(b)). Occurrence is the ratio of the number of samples containing the FN form to the total number of samples. The relative amount of the FN band is the percentage of the total number of pixels found in the electrophoresis path.