DHM reduced LPS-induced oxidative stress through inhibiting the production of reactive oxygen species (ROS) and enhanced the antioxidant system by activating superoxide dismutase (SOD) and the Nrf2/HO-1 pathway
Xuejun et al. (2018)
HepG2 cells
Reactive oxygen species (ROS)
DHM at 10, 50, or 100 mM for 6 h, 12 h, and 24 h
DHM reduced ROS accumulation in a concentration-dependent manner in HepG2 cells
Bin et al. (2014)
Ampelopsin purity 95%
In vitro assays
DPPH, ABTS, H2O2, and O2 radical methods
37°C for 15, 20, and 60 minutes
DHM inhibited free radicals. values of DHM for scavenging DPPH, ABTS, H2O2, and O2 radicals were 8.18, 5.32, 7.95, and 7.79 (μg/mL), respectively
Xiang et al. (2017)
Ampelopsin 98%
PC12 cells
Reactive oxygen species
Ampelopsin at 50 and 100 μM for 1 h
DHM inhibited reactive oxygen species in 6-OHDA stimulated PC12 cells in concentration-dependent manner
Xianjuan et al. (2015)
Glomerular mesangial cells (MCs)
ROS and ROS enzymes
DHM at 0, 10, 20, and 40 μM for 24 h
DHM inhibited the intracellular ROS production and expression levels of ROS-producing enzymes NADPH oxidase 2 (NOX2) and NOX4 and mediated the antioxidative effects through the activation of Nrf2/HO-1 pathway
Chunping et al. (2020)
In vitro assays
Hydroxyl and superoxide radical methods
Ampelopsin 10 to 100 μM for 60 min and 25°C for 20 min
Ampelopsin eliminated •OH and O2•– in a concentration-dependent manner; the values were and
Jiantao et al. (2008)
PC12 cells
Reactive oxygen species
Ampelopsin at 1, 5, and 15 mg/mL for 1 h
Inhibited the formation of reactive oxygen species (ROS) and enhanced the cellular antioxidant defense through activation of the ERK and Akt signaling pathways in PC12 cells
Xianjuan et al. (2011)
DHM 64.7%
In vitro assay
DPPH
DHM at 2, 4, 6, 8, 10, and 12 ppm for 30 min
DHM extract inhibited DPPH radicals with value of 3.9 ppm
Liyun et al. (2015)
In vitro assays
DPPH and ORAC
DHM at 12.5, 25, 50, 100, 200, and 400 μg/mL for 30 min
DHM dose-dependently inhibited the DPPH and ORAC radicals
Kun et al. (2019)
HepG2 cells
Nrf2/Keap1 pathway
40 μM for 3-12 h
Exhibited antioxidant activity by activating the cellular Nrf2/Keap1 pathway
Kun et al. (2019)
Not provided
(HEI-OC) 1 auditory cells
ROS
DHM at 10, 100, and 1000 μM for 24 h
DHM inhibited ROS accumulation in HEI-OC cells
Hezhou et al. (2020)
B16F10 mouse melanoma cells
Reactive oxygen species
1, 25, and 50 μM for 24 h
DHM reduced intracellular reactive oxygen species and reactive species (RS) levels
Huey-Chun et al. (2016)
Rat cardiac fibroblasts
Ang II-induced oxidative stress
0–320 μM for 4 h or 80 μM for 0–24 h
DHM inhibited cellular reactive oxygen species production and MDA level, and enhanced the SOD activity and total antioxidant capacity (T-AOC)
Qiuyi et al. (2017)
In vitro assays
DPPH and ABTS
100 mg/mL for 6 min or 30 min
Cookies fortified with DHM significantly enhanced the DPPH and ABTS radical scavenging activities
Jing et al. (2018)
DHM 97%
PC12 cells
Methylglyoxal- (MG-) induced oxidative stress in PC12 cells
20 and 10 mol/L for 24 h
Inhibited the intracellular ROS and modulating AMPK/GLUT4 signaling pathway in PC12 cells
Baoping et al. (2014)
DHM 95%
In vitro assay
DPPH
0 to 50 μg/mL for 30 min
DHM and lecithin complex inhibited DPPH radicals with value of 22.60 μg/mL
Benguo et al. (2009)
Rat cardiomyocytes
Ang II-stimulated reactive oxygen species in cardiomyocytes
20, 40, 80, and 160 μM for 8 h, 12 h, 24 h, or 48 h
DHM reduced ROS generation in Ang II-stimulated cardiomyocytes by increasing total antioxidative capacity through upregulating expression of SOD and thioredoxin
DHM reduced ROS overproduction, decreased MDA level and increased SOD activity and showed antioxidant activity in HUVECs by activating the PI3K/Akt/FoxO3a signaling pathway
Xiaoying et al. (2019)
DHM 99%
Human umbilical vein endothelial cells (HUVECs)
H2O2-induced oxidative stress
37.5-300 μM for 2 h
DHM inhibited intracellular ROS overproduction in HUVECs cells
Xiaolong et al. (2015)
PK-15 cells
H2O2-induced oxidative stress in PK-15
0, 15, 30, and 60 μg/mL for 1 h
Significantly decreased MDA production in H2O2-induced PK-15 cells
Tan et al. (2010)
DHM 98%
Colo-205 cells
Reactive oxygen species and MDA
8, 16, and 32 μM for 2 h
Inhibited reactive oxygen species and malondialdehyde levels
Jun et al. (2019)
Ampelopsin 98%
In vitro assay
DPPH method
0.1 to 0.4 μg/mL for 60 min
DHM inhibited DPPH radicals with value of 0.235 μg/mL
Wenzhen et al. (2014)
DHM 98%
In vitro assays
DPPH, ABTS, O2 radical, and Fe2+ chelating method
2 to 20 μg/mL for 30 min
Eliminated ABTS, DPPH free radicals, reduced Cu2+, and chelated Fe2+
Xican et al. (2016)
C57BL/6J mice
Streptozotocin-induced oxidative stress model
DHM at 100 mg/kg/day for 14 weeks
DHM decreased MDA and increased the SOD and GSH-Px
Bin et al. (2017a)
DHM (purity not provided)
Male C57BL/6 mice
Transverse aortic constriction induced oxidative stress in mice
DHM (250 mg/kg/day) for 2 weeks
DHM administration reduced reactive oxygen species and malondialdehyde level, and increased total antioxidant capacity and SOD activity in mice
Yun et al. (2018)
Chickens
LPS-induced oxidative stress in chickens
0.025%, 0.05%, and 0.1% for 14 days
DHM increased SOD and GSH-Px activity and GSH in chicken plasma and ileum
Yicong et al. (2020)
DHM (purity not provided)
ICR mice
Sleep deprivation induced oxidative stress
100, 50, and 25 mg/kg/day for 14 days
DHM increased SOD activity and reduced MDA level
Hongxiang et al. (2019)
DHM (purity not provided)
Rat
DPPH radical scavenging activity of rat serum
100 mg/kg/BW
DHM increased the antioxidative capacity of rat serum against DPPH radicals
Xiao et al. (2014)
Ampelopsin 95%
Piglets
LPS-induced oxidative stress in piglets
2.5, 5, and 10 μg/mL for 30 min
Decreased the MDA and protein carbonyl levels in LPS-treated piglets
