Mulberry Juice-Derived Carbon Quantum Dots as a Cu<sup>2+</sup> Ion Sensor: Investigating the Influence of Fruit Ripeness on the Optical Properties

Salih Ajaj, Chiayee; Sadiq, Diyar

doi:https://doi.org/10.1155/2023/9980479

Nanomaterials and Nanotechnology

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Abstract Introduction Materials and Methods Results and Discussion Conclusion Data Availability Conflicts of Interest Authors’ Contributions Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2023 | Article ID 9980479 | https://doi.org/10.1155/2023/9980479

Mulberry Juice-Derived Carbon Quantum Dots as a Cu²⁺ Ion Sensor: Investigating the Influence of Fruit Ripeness on the Optical Properties

Chiayee Salih Ajaj¹and Diyar Sadiq¹

Academic Editor: Raphael Schneider

Received06 Jul 2023

Revised05 Sept 2023

Accepted07 Sept 2023

Published20 Sept 2023

Abstract

This study synthesized carbon quantum dots (CQDs) with green photoluminescence through a hydrothermal method that utilized mulberry juice as the carbon source. The influence of fruit ripeness on the physical and chemical properties, focusing on the fluorescence spectra, has been explored. Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive X-ray analysis (EDX) showed that there were oxygen-containing groups, and X-ray diffraction (XRD) showed that the carbon quantum dots (CQDs) were graphitic. The results revealed that the CQDs had an average size of around 7.4 nm and 9.7 nm for unripe and ripe mulberry juice, respectively. These CQDs emitted green light at 500 nm and 510 nm in unripe and ripe mulberry juice, respectively, when excited at a wavelength of 400 nm. The prepared CQDs exhibited excitation-dependent photoluminescence (PL) emission behavior, demonstrating their dependence on the excitation light. The impact of fruit ripeness on optical properties was explored by examining fluorescent spectra from different fruits (including tomato and blackberry), demonstrating comparable behaviors observed in mulberry fruit. In addition, the prepared CQDs were utilized as a fluorescent sensor with high specificity to detect Cu²⁺ ions. The detection limit (DL) for this sensor was determined to be 0.2687 µM, and the limit of qualification (LOQ) is 0.814 µM. The linear range for detection lies between 0.1 and 1 µM. The selectivity of the CQDs towards Cu²⁺ ions was confirmed by recording the PL response for Cu²⁺ ions compared to the weak response of other metal ions. According to these results, the CQDs can be applied in various cellular imaging and biology applications, bio-sensing, optoelectronics, and sensors.

1. Introduction

Carbon quantum dots (CQDs) are a class of quantum dots (zero-dimensional nanostructures) that have exclusive properties, consisting of quantum energy levels and excellent optical and electrical properties [1]. The unique characteristics of CQDs include low toxicity, good biocompatibility [2], high water solubility, excellent optical properties, catalytic properties, electrical conductivity, high stability, and easy functionalization that are responsible for their utility in a wide range of applications such as bio-imaging [3], cellular imaging [4], metal sensing [5], drug delivery [6], energy storage [7], optoelectronics [8], sensors [9], light-emitting diode (LED) [10], and antibacterial and tissue engineering [11–17]. Though there has been fast growth in the area of CQDs, their synthesis is pretty challenging due to difficult reaction conditions, damaging starting materials, and steps in surface passivation [11].

To date, the synthesis of CQD has been carried out using natural and renewable green materials. These sources are used to create fluorescent CQDs. The dopant particle size, the dopant’s desire, the impact of the passivating agent, pH [5], time, temperature [18], and the nature of the solvent are critical in affecting how the CQDs behave fluorescently. When electron-rich heteroatoms such as nitrogen, boron, and sulfur are doped on the surface of the CQDs, their capacity to fluoresce and their catalytic behaviors are considerably modulated [14]. Moreover, the created CQDs can also be used as efficient fluorescent probes for sensing, catalysis, and biological applications [19, 20].

Nowadays, because they are cheap, abundant, easy to find, and environmentally beneficial, CQD precursors made of green natural materials are becoming increasingly attractive as carbon sources [21]. CDs are a class of quantum dots that can be created from several carbon-based precursors. Numerous studies have demonstrated CQDs with carbon sources from fruit-derived sources, including Carica papaya [22], tomato [23], pear, avocado, kiwi [24], tapioca [25, 26], lemon, from perennial grass such as sugarcane [27, 28], and oranges such as satang honey, lime, mandarin, and sweet orange [21]. The techniques for creating CQDs that are most frequently utilized are chemical oxidation, ultrasonic synthesis, hydrothermal synthesis, solvothermal synthesis, microwave synthesis, and laser ablation. Among them, the hydrothermal technique is the most facile approach to the green process because it uses a soft chemical pathway and a one-step synthesis which makes it inexpensive, simple, and affordable and has mild reaction conditions and is environmentally benign. It produces CQDs with uniform particle sizes, making them great candidates for use in many applications. However, despite the extensive effort put into the green synthesis of CQDs, the effect of plant ripeness remains unstudied.

Research in CQD has become increasingly focused on practical applications. Advances have been made in areas such as optoelectronics, catalysis, and sensing. Among these applications, metal ion detection has emerged as the most popular and easily achievable. Heavy metal pollutants, like copper, lead, and cadmium, are a major concern for water quality and human health worldwide. Carbon quantum dots (CQDs) offer a promising solution for the efficient detection and removal of heavy metals due to their small size, surface area, and functional groups. CQDs from natural sources, such as fruit extracts, have shown success as fluorescence sensors for heavy metal ions. Mulberry fruit-, papaya- [29], and tapioca-derived [26, 30] CQDs offer a sustainable and creative option for addressing water contamination.

Herein, we present a comprehensive study on the biosynthetic carbon quantum dots (CQDs) obtained from mulberry fruit via a hydrothermal method, focusing on their synthesis, characterization, and potential application as a highly selective sensor for Cu²⁺ ions. More importantly, the research investigates the influence of fruit ripeness on the physical and chemical properties of the obtained CQDs, which, to the best of our knowledge, has not been explored before. Extensive studies of the fluorescence properties of the obtained samples revealed different physical and chemical behaviors between CQDs obtained from ripe and unripe sources. The difference in fluorescence spectra of CQDs between ripe and unripe fruit was also observed in other fruits, such as tomatoes and blackberries. In addition, we investigated the effect of pH on the fluorescence intensity of the CQDs. Furthermore, the selective detection of Cu²⁺ ions utilizing mulberry-based CQDs with adjustable optical characteristics opens the door to the creation of highly sensitive and specific fluorescence sensors in a variety of applications. These intriguing results shed light on the relationship between fruit ripening and the emission behavior of CQDs and reveal factors such as size and molecular density changes that lead to this phenomenon.

2. Materials and Methods

2.1. Experimental Materials and Reagents

Fresh mulberries were prepared from homemade trees. Since they were all of the reagent quality, they were all used without additional purification. The deionized water was made in the chemistry laboratory.

2.2. Instrumentation and Characterization

Transmission electron microscopy (TEM) was carried out utilizing a TEM TEC9G20, FIE, USA [31]. Photoluminescence spectroscopy was performed using a homemade fluorescence spectrophotometer (tunable light source and spectrometer from SCEINCETECH 9702 (Canada)). The high-resolution X-ray diffraction (HR-XRD) was performed using a (PAN X-Pert) from the United Kingdom. Fourier transform infrared (FT-IR) spectra were taken using (Thermo-Scientific, USA). UV-Vis’s absorption spectra have been recorded on a CECIL CE 7200. The 7000 series UV-Vis’s measures wavelength between 190 nm and 900 nm, and an EDX instrument was used to identify the elemental composition of materials using a (QUANTA 450, Carl Zeiss Ag (supra 55VP) using an acceleration voltage of 5–30 Kv). A KT7-900-434 highspeed centrifuge was used from Heller International Trading Co., Ltd (Kenda, Germany). A DW-120D ultrasonic cleaning machine was used (Ultrasonic cleaner, China), and an AL104 electronic balance from the Mettler Toledo Instrument Co., Ltd. (Shanghai, China) was used. Finally, a high-temperature muffle furnace was taken using a DRAWELL furnace, in Shanghai, China.

2.3. Preparation of CQDs

The preparation process for synthesizing CQDs is illustrated in Figure 1. The CQDs were synthesized through a single-step solvothermal approach, employing mulberry juice as the carbon source and deionized water as the organic solvent. The procedure consists of several sequential stages. First, mulberry juice was extracted from fragmented fruit and filtered using a 0.22 µL filter. The resulting juice was centrifuged for 10 minutes at 9000 rpm to separate pulp-free mulberry juice from the supernatant. After 15 minutes, a mixture of 20 mL of filtered mulberry juice and 20 mL of deionized water was stirred. The resulting mixture is placed in a 200 mL Teflon-lined steel autoclave and heated for six hours at 180°C by the hydrothermal synthesis method [32]. After cooling the system to ambient temperature, the resulting brown products are centrifuged at 14,000 revolutions per minute for 10 minutes. The products were then subjected to freeze drying, and further testing was conducted.

2.4. Fluorescence Detection of Cu²⁺ Ions

The measurement of Cu²⁺ was conducted in a water-based solution under ambient conditions. In a standard experiment, the stock solutions of Cu²⁺ ions (100 mM) were generated through the process of dissolving and diluting Cu²⁺ in deionized water. To verify the presence of Cu²⁺ ions, a series of solutions with different concentrations of copper solution are mixed with a consistent volume of 1.0 mL of CQD solution. The fluorescence spectra were recorded after 2 minutes. Samples of water for the spike recovery experiment were obtained from the laboratory tap water source.

3. Results and Discussion

3.1. Structure and Morphology of CQDs

The degree of carbon ordering and crystallinity of two varieties of ripe and unripe mulberries were investigated using XRD. The XRD pattern for ripe mulberry at 180 C° is shown in Figure 2(a). The typical peak of ripe CQDs is at =21.93° which corresponds to the (002) plane with a d-spacing of 0.405 nm and can be found in the carbon material [33, 34]. The second peak is seen at 42.0498° and 49.21°, indicating the crystalline graphitic structure of the C (100) plane of the CQDs [35]. The obvious unripe CQDs’ broad peak center around 20.5°, as illustrated in Figure 2(b), indicates highly disordered carbon atoms with a high degree of C (002) that still contain oxygen-containing functional groups in the CQDs. In addition, the second peaks of unripe CQDs are seen at 41.996°, 44.58°, and 48.92°, also indicating the crystalline graphitic structure of the C (100) plane of the QCDs. When comparing the ultraviolet (UV) absorption properties of ripe and unripe CQDs, a notable observation is a slight blue shift, indicating a decrease in absorption intensity. Moreover, from the results of improving the optical properties of CQDs, it has been observed that ripe CQDs exhibit greater significance compared to unripe CQDs.

(a)

(b)

The shape and size of the synthesized mulberry samples were explored by TEM. The TEM picture of ripe mulberry shows that the arranged CQDs were monodispersed and quasi-spherical with an estimated dispersion extending from 6 to 12 nm and an average diameter of 9.7 nm, as shown in Figure 3(a). The TEM picture of unripe mulberry shows that the prepared CQDs were monodispersed and quasi-spherical with dimension distribution ranging from 6 to 12 nm and a common diameter of 7.4 nm, as shown in Figure 3(b).

(a)

(b)

Energy dispersive X-ray (EDX) is a technique that reveals the presence of elements present in the materials. We use this technique to find the presence of materials in unripe and ripe mulberries. The quantity of each element that EDX found was applied, as shown in Figure 4. The EDX spectra of the synthesized ripe CQDs mostly contain carbon (72%), and oxygen (26%), and unripe CQDs mostly contain carbon (69.48) and oxygen (26%). Even after the reduction of CO, the existence of O may be caused by the presence of oxygen-containing functional groups. In addition, the presence of Au is for the coating by the gold, and Cu is the stick copper that makes it stick between the samples and the sample holder. According to the EDX spectrum, there is a larger concentration of C than O [36]. The presence of a significant concentration of oxygen elements in the adsorbent results in improved adsorption efficiency due to the strong tendency of the interfacial area to include a higher number of disrupted π–π bonds. The colloidal quantum dots (CQDs) exhibited a significant degree of oxidation due to the elevated presence of oxygen atoms, thereby leading to the formation of a greater number of reactive sites on their surface.

(a)

(b)

3.2. Surface Chemistry Composition Analysis

FT-IR spectroscopy was used to examine the chemical structure and makeup of CQDs, as illustrated in Figure 5. For the pure ripe and unripe mulberry powder, the clear broad peaks at 3236 cm⁻¹ ripe mulberry and 3294 cm⁻¹ unripe mulberries correspond to the O-H stretching vibration and the absorption bands at 2885 cm⁻¹ and 2098 cm⁻¹ had been attributed to the C=C stretching vibration [1, 37]. The absorption band at 1631 cm⁻¹ for both types of mulberries was identified as the C=O bending vibration. The peaks at 1192 cm⁻¹ and 1519 cm⁻¹ were due to the C=C stretching vibrations. The peaks at 976 cm⁻¹ and 980 cm⁻¹ were attributed to the vibrations of C-H bonds. The peaks at 671 cm⁻¹ and 663 cm⁻¹ were due to C-H bonds, respectively. Compared to mulberry, CQDs showed an obvious minimization in the absorption of O-H stretching vibrations at 3236 cm⁻¹ for ripe mulberry and 3294 cm⁻¹ for unripe mulberry, and C-H vibrations at 671 cm⁻¹ for ripe mulberry and 663 cm⁻¹ for unripe mulberry. Moreover, the three clear absorption peaks at 3236, 1631, and 671 cm⁻¹ are related to the stretching and bending vibrations of O-H, C=C, and C-H, respectively. FT-IR analysis validated the existence of hydroxyl and carbonyl functionalities on the surface of CQDs.

3.3. Optical Properties of CQDs

The most useful method for examining the optical properties of CQDs is UV-vis spectroscopy. The UV-Vis spectrum of the CQDs was found to have one characteristic absorption peak located at wavelengths of 243 nm for ripe CQDs and a second peak located around 282 nm for unripe CQDs in the spectrum as illustrated in Figure 6. These peaks are associated with a π–π transition of aromatic -C=C- bonds aromatic sp² hybridization and n–π transition of the conjugated C=O band which is similar to early reports [36, 38, 39]. The progressive increase in unripe CQDs results in a shift towards longer wavelengths in the absorption, indicating the occurrence of ground-state complex formation in CQD.

The PL emission spectra properties of the CQDs were investigated. It is well known that the PL emission intensity of the CQDs is highly dependent on the excitation wavelengths [40, 41]. Figure 7 presents the feature of excitation-dependent emission spectra showing different maximum emission wavelengths under the variation of the excitation wavelength changing from 350 to 450 nm for the ripe and unripe mulberry. The CQDs excited at 400 nm show strong yellow PL located at 500 nm for unripe mulberry and 510 nm for ripe mulberry; this behavior is frequently observed in some carbon dots and can be attributed to different emissive states present on the surface of carbon dots. When the excitation wavelength was changed from 350 to 450 nm, the PL intensity gradually increased due to the graphitic carbon cores of the CQDs transitioning from π to π, and the PL emission peaks were significantly red-shifted, as shown in Figures 7(a) and 7(b). The result reveals that CQDs show different optical properties based on their ripeness. This may be anticipated to be caused by variations in their molecular densities and other chemical characteristics, such as functional groups [42, 43].

(a)

(b)

To understand the role of ripe and unripe plants on their optical properties and to find whether the ripeness effect on optical properties may only occur in mulberry fruit, PL spectra from other two green sources (tomatoes and blackberries) were tested, as displayed in Figure 8. The PL properties of both green source materials showed a similar trend as the mulberry fruit and as expected exhibited excellent excitation light-dependent photoluminescence (PL) emissions. However, interestingly, the PL spectra of the unripe and ripe parts of both plants exhibited a notable shift. The results showed that the maximum PL spectra of the ripe tomato CQDs (Figure 8(a)) were located at 503 nm under a 410 nm wavelength excitation, while the maximum PL spectra of the unripe tomato CQDs (Figure 8(b)) was at 490 nm under a 400 nm wavelength excitation. A similar trend of emission properties was found for the blackberry CQDs, and the results showed maximum PL spectra of the ripe blackberry CQDs (Figure 8(c)) at 523 nm under a 490 nm wavelength, while the maximum PL spectra of the unripe blackberry CQDs (Figure 8(d)) were located at 492 nm under a 400 nm wavelength. This result specified that the plant’s ripeness indeed slightly modulates its optical properties. This could be due to the different sizes of the CQDs for ripe and unripe fruit [27].

(a)

(b)

(c)

(d)

4. Detection of Cu²⁺

The sensing characteristics of CQD detection systems are critically dependent on selectivity. To validate the selectivity of the CQDs to Cu²⁺ ions, the fluorescence intensity of CQDs solutions in the presence of various metal ions (Ag⁺, Fe³⁺, Zn²⁺, Pb²⁺, Cd²⁺, Co²⁺, Na⁺, K⁺, Hg²⁺, Cu²⁺, Cl⁻, SO₄²⁻, and NO₃⁻) was explored. The fluorescence response of the CQDs solution towards various metal ions each at a consistent concentration of 100 mM was recorded at an excitation wavelength of 370 nm. Figure 9 displays the behaviors of the fluorescence quenching of the various metal ions. Cu²⁺ ions addition showed a remarkable effect on the CQDs fluorescence response compared to other metal ions that showed a negligible effect on fluorescence intensity. These results revealed that the prepared CQDs could be successfully used to selectively detect Cu²⁺ ions by recording the fluorescence intensity. Cu²⁺ ions can specifically coordinate with the phenolic hydroxyl groups on the surface of CQDs to quench their fluorescence [44–46].

The effective energy transfer between Cu²⁺ and the groups on the surface of the CQDs may be the cause of the fast fluorescence quenching, as shown in Figure 10.

It becomes clear that CQDs can be used as a sensitive fluorescence sensor for Cu²⁺ ion detection (Table 1). The sensitivity of the prepared CQDs for the determination of Cu²⁺ ions was also investigated. As the concentration of Cu²⁺ ions increased, the fluorescence intensity of the carbon quantum dots CQDs was observed to gradually decrease, as depicted in Figure 11. Consequently, the ratio of the fluorescence intensity (I) to the initial fluorescence intensity (I₀), denoted as I/I₀, decreased linearly with the concentration of Cu⁺² ions in the range 0.1 to 1 µM, displaying a strong linear correlation with a coefficient of determination (R²) of 0.9864, as illustrated in Figure 11. The detection limit was 0.2687 µM, and the limit of qualification (LOQ) is 0.814 µM, indicating that the CQDs pose potential utility to be used as a fluorescence sensor for Cu²⁺ ions detection [45].

(a)

(b)

(c)

5. Conclusion

This study successfully created green luminescent carbon quantum dots (CQDs) through a simple hydrothermal method, using mulberry fruit as the carbon source. The physical and chemical properties of the CQDs were investigated, revealing that fruit ripeness directly impacted the fluorescence spectra and characteristics of the CQDs. The fluorescence spectra and high-resolution transmission electron microscopy (HR-TEM) images showed that the average diameter and intensity of photoluminescence (PL) of CQDs changed depending on how ripe the fruit was. Under 400 nm excitation, the PL peak was 510 nm for ripe and 500 nm for unripe mulberries, with corresponding CQD sizes of 9.7 and 7.4 nm, respectively. The CQDs also showed excitation-dependent PL emissions due to surface state defects. Energy-dispersive X-rays (EDX) and X-ray diffraction (XRD) showed that the CQDs made in the laboratory were graphitic and had hydroxyl and carboxyl groups on the surface. These groups are mostly made of carbon and oxygen. Fourier-transform infrared (FT-IR) analysis revealed that the surface functional groups consisted of O-H, C=O, and C=C functional groups. The synthesized CQDs demonstrated exceptional selectivity and sensitivity towards Cu⁺² ions, with a detection limit (DL) of 0.2687 µM and a limit of qualification (LOQ) of 0.814 µM. A strong linear relationship between Cu²⁺ ion fluorescence intensity and concentrations within the 0.1–1 µM range was established through fluorescence quenching, demonstrating its capacity for sensitive Cu²⁺ ion detection. These CQDs are a promising solution for fluorescence-based detection applications because of their easy and cost-effective synthesis.

Data Availability

All underlying data are available as part of the article and no additional source data are required.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Authors’ Contributions

Chiayee Salih Ajaj investigated (equal) the study, designed a methodology (equal), and wrote, reviewed, and edited (equal) the manuscript. Diyar Sadiq designed a methodology (equal), supervised the study (equal), and wrote, reviewed, and edited (equal) the manuscript.

Acknowledgments

The authors would like to thank Mr Shinwar A. Idrees for his support.

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Copyright © 2023 Chiayee Salih Ajaj and Diyar Sadiq. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nanomaterials and Nanotechnology

Mulberry Juice-Derived Carbon Quantum Dots as a Cu2+ Ion Sensor: Investigating the Influence of Fruit Ripeness on the Optical Properties

Abstract

1. Introduction

2. Materials and Methods

2.1. Experimental Materials and Reagents

2.2. Instrumentation and Characterization

2.3. Preparation of CQDs

2.4. Fluorescence Detection of Cu2+ Ions

3. Results and Discussion

3.1. Structure and Morphology of CQDs

3.2. Surface Chemistry Composition Analysis

3.3. Optical Properties of CQDs

4. Detection of Cu2+

5. Conclusion

Data Availability

Conflicts of Interest

Authors’ Contributions

Acknowledgments

References

Copyright

Mulberry Juice-Derived Carbon Quantum Dots as a Cu²⁺ Ion Sensor: Investigating the Influence of Fruit Ripeness on the Optical Properties

2.4. Fluorescence Detection of Cu²⁺ Ions

4. Detection of Cu²⁺