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Neural Plasticity
Volume 2015 (2015), Article ID 256389, 12 pages
Research Article

Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain

Department of Anatomy and Cell Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan

Received 27 May 2015; Revised 3 September 2015; Accepted 14 September 2015

Academic Editor: Jessica C. Kwok

Copyright © 2015 Noriko Horii-Hayashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

WFA staining was performed using sections pre-treated with ChABC to confirm the specificity of WFA binding. This treatment almost completely abolished WFA reactivity in the sections of the primary somatosensory cortex (S1, Fig. S1A), piriform cortex (Pir, Fig. S1B), reticular thalamic nucleus (Rt, Fig. S1C), hippocampus (Hc, Fig. S1D), ventromedial hypothalamic nucleus/arcuate nucleus (VMH/Arc, Fig. S1E), inferior colliculus (IC, Fig. S1F), and gigantocellular reticular nucleus (Gi, Fig. S1G) at 11w. However, small dot-like reactivity was observed in sections of the Hc at P7 after the same treatment (Fig. S1H). Such dot-like WFA reactivity was widely and frequently observed in immature brain sections and considered a false positive reaction of WFA.

  1. Supplementary Material