Increases in Retrograde Injury Signaling Complex-Related Transcripts in Central Axons following Injury

Figure 6

Inhibition of nuclear transport results in downregulation of axonal transcriptional changes. Real-time RT-PCR was used to measure levels of axonal mRNA in injured hippocampal axons after inhibiting nuclear transport with IPZ. IPZ treatment suppresses elevated importin 1, RanBP1, vimentin, and -actin mRNA levels (white bars). Grey bars (previously graphed in Figure 2, 24 hrs) indicate untreated axons and are included for clarity of comparison. Error bars represent the SD. indicates significant difference of IPZ treated and untreated cells from their corresponding controls (p < 0.05) based on pairwise fixed allocation test. for all groups, except for Ranb1 (see Section 2).