Identification of Binding Partners of Deafness-Related Protein PDZD7

Figure 4

Interaction of PDZD7 with CADM1 in transfected cells. Expression vectors were transfected into COS-7 cells, and the subcellular localization of target proteins was determined using confocal microscopy. (a) EGFP-CADM1 localized in the cytoplasm of COS-7 cells. (b) When expressed together, PDZD7-mCherry colocalized with EGFP-CADM1. Scale bar: 10 μm. (c) Western blots showed that CADM1 was coimmunoprecipitated with PDZD7. Expression vectors were transfected into HEK293T cells to express epitope-tagged PDZD7 and CADM1 proteins, and cell lysates were subjected to immunoprecipitation. IP indicates antibody used for immunoprecipitation, and WB indicates antibody used for detection.

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