Blocking Epidermal Growth Factor Receptor Signaling in HTR-8/SVneo First Trimester Trophoblast Cells Results in Dephosphorylation of PKB𝜶/AKT and Induces Apoptosis

Table 1

Kinexus quantitation of phosphosites in response to Iressa and EGF. Table shows counts/minute/ at each phosphorylation site in control cells compared to EGF, EGF + Iressa-treated cells, and Iressa. These data are the quantitative results from the immunoblots in Figure 1.

Phosphoprotein	Control; CPM	EGF; CPM	Iressa + EGF; CPM	Iressa CPM
Adducin a	2525.76	3230.00	2152.00	2589.00
Adducin g	1661.92	1457.00	1969.00	596.00
CDK1	4850.79	3184.00	4417.00	3311.00
CREB	412.29	525.00	1138.00	535.00
ERK1	1620.04	1394.00	1186.00	179.00
ERK2	1051.38	1175.00	913.00	186.00
GSK3a	186.75	189.00	147.00	165.00
GSK3a	3476.29	3194.00	1829.00	3144.00
GSK3b	2330.41	2620.00	2381.00	3279.00
GSK3b
JUN	289.71	432.00	612.00	801.00
MEK1/2	538.75	522.00	460.00	136.00
MEK3/6	3114.65	2480.00	2341.00	3078.00
MEK6
MSK1/2
MSK1/2	284.33	378.00	271.00	337.00
NR1	752.75	351.00	399.00	196.00
p38 MAPK	2201.98	5045.00	2331.00	437.00
p70 S6K		248.00
p70 S6K	859.82	1218.00	777.00	866.00
PKBa (S473)	441.27	1887.00	243.00	326.00
PKBa (T308)		415.00
PKCa	9767.38	12176.00	14306.00	14817.00
PKCa/b	4652.87	5744.00	8439.00	8680.00
PKCd
PKCe	1533.96	1475.00	1151.00	1382.00
PKR
RAF1	2223.67	1443.00	1289.00	1307.00
RAF1	5897.68	4372.00	4195.00	3547.00
RB	795.37	1314.00	411.00	1116.00
RSK1
SAPK	285.45	153.00	179.00	145.00
SMAD1
SRC
STAT1	3071.94	3942.00	6013.00	3613.00
STAT3
STAT5	10297.67	14160.00	11465.00	10810.00