Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium
Figure 6
Decomposition of SIN-1 in FCS/RPMI. (a) Confirmation of SIN-1 decomposition. SIN-1 (1 mM) dissolved in FCS/RPMI was decomposed aerobically in a CO2 incubator for 2-3 h. At the indicated time points, serum proteins were precipitated with PCA, and samples were analyzed by HPLC, as described in Figure 2. The areas for SIN-1 at 0 h and SIN-1C at 3 h were taken as 100%. Values are means ± ranges of two independent experiments. (b) NO release from residual SIN-1 in the medium. The aliquot was diluted 10-fold with PBS, and NO levels were measured by an NO electrode. Where indicated by the arrows, CuSO4 was added to a final concentration of 1 mM to oxidize SIN-1.
