Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium
Figure 8
Effects of FCS, BSA, and Cys on the cytotoxicity of decomposed SIN-1 in cell culture media. (a) Effect of FCS on the cytotoxicity of decomposed SIN-1. SIN-1 (1 mM) was decomposed in either 10% FCS/RPMI, BSA (3 mg/mL)/RPMI, or RPMI for 2 h as in shown in Figure 7, followed by the addition of fresh FCS at a final concentration of 10% and exposure to cells at 100 L/well. Viability was measured 24 h after exposure. (b) Comparison of the protective effects of BSA and NEM-BSA against the cytotoxicity of 2 h SIN-1-decomposed FCS/RPMI. Cells were treated with 2 h SIN-1-decomposed FCS/RPMI (1 mM), together with BSA or NEM-BSA (3 mg/mL). (c) Effect of Cys on the cytotoxicity of decomposed SIN-1. As in (b), cells were treated with 2 h SIN-1-decomposed FCS/RPMI (1 mM) together with the indicated concentrations of D- or L-cysteine. All values are means ± intra-assay deviations, expressed as SD, from four or five wells in a representative experiment. .
