Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?
Figure 5
Control experiments for excision activities measured by the experiment described in Figure 4(a). (a) Nucleotide excision activities in some nuclear extracts of lymphocytes from the 24 blood sample. Nuclear extracts which failed to excise amoxicillin-induced DNA lesions were selected and used for examining their activities to excise UV-induced lesions. To determine the nucleotide excision activity, AGS cells treated with 5 J/m2 UV were used as substrates in comet-NE assay. (b) Complementation test. Nuclear extracts samples from Helicobacter pylori carrier (HP) and HP-HBV (HP-HB) double carrier were mixed with the NE from p53 deficient cell line H1299 and used as in comet-NE assay. The NE from H1299 cell and healthy (H) people were severed as negative and positive control.
