Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?
Figure 4
Variation of excision activities in nuclear extracts of lymphocytes from 24 blood samples. (a) To test the lymphocyte’s nuclear etract’s (NE’s) activity, the AGS cells were served as substrate. AGS cells were treated with 5 mM amoxicillin to induce oxidative DNA damage and examined by comet-NE assay with lymphocyte’s NE. The sample code was given according to those described in Figure 2, and the carriers and noncarriers of HP or HBV are indicated with + or −, respectively. (b) Correlation between the oxidative DNA damage levels of Figure 2 and the excision activities of Figure 4(a) (note: samples coded 2, 13 and 18 were excluded from the correlation because the concern of artifact due to sample preparation; their respective NER activities shown in Figure 5(a) are also low). The value (= −0.30), the correlation coefficient, was obtained from statistical analysis.
