Mitochondrial Dysfunction Induces Formation of Lipid Droplets as a Generalized Response to Stress
A defect in hepatic LD formation by CLP in heme oxygenase (HO)-1-deficient mouse. Polymicrobial sepsis was induced as described in Figure 4 in HO-1-null and control mice. Very few LDs were observed in both wild-type (a) and HO-1-null mice (b). The expression of LD coat proteins and caveolin-1 was examined (c). FAS levels are generally unaltered in the absence of HO-1 (d). Measurement of basal levels of caveolin-1 and validation of HO-1-knockout mice were carried out with both cell lysates (e). β-Actin blot serves as a loading control.