Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 5

Research Article

Tanshinol Attenuates the Deleterious Effects of Oxidative Stress on Osteoblastic Differentiation via Wnt/FoxO3a Signaling

Figure 5

Tanshinol rescues oxidative stress-elicited inhibition of Wnt/β-catenin signaling. (a) C2C12 cells were treated as described in Figure 4, and protein levels of β-catenin were detected by Western Blot. Representative immunoblots were shown in upper panel. Quantitative results of relative band intensities of protein are showed in lower panel. (b) C2C12 cells were transfected with the Tcf-luc reporter plasmid or negative control. Cells transfected were treated with or without Tanshinol in the presence or absence of Wnt3a for 1 h, followed by vehicle control, H2O2 for 24 h. Luciferase activity assays were explored as described under “Section 2”. The data represent mean ± SEM of luciferase relative luminescence units (RLU) normalized to corresponding Renilla luciferase activity (triplicates). (c) C2C12 cells were treated as described in Figure 4, and the expression levels of Axin2, ALP, and OPG mRNA were quantified by qRT-PCR and normalized to GADPH mRNA. Note: (1) Con (vehicle control); (2) H (H2O2); (3) T (Tanshinol); (4) H + T (H2O2 + Tanshinol); (5) H + R (H2O2 + Resveratrol). Error bars indicate mean ± SEM of at least three independent experiments. versus vehicle control and versus H2O2 treatment.

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