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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 438321, 8 pages
http://dx.doi.org/10.1155/2013/438321
Clinical Study

Effects of Open versus Laparoscopic Nephrectomy Techniques on Oxidative Stress Markers in Patients with Renal Cell Carcinoma

1The Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland
2Department of Tissue Engineering, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland
3Department of Neurosurgery, Stanisław Staszic Specialist Hospital, Rydygiera 1, 64-920 Piła, Poland
4Department of Normal Anatomy, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland
5Department of Nephrology and Internal Medicine, City Hospital, Szpitalna 19, 85-826 Bydgoszcz, Poland

Received 19 December 2012; Accepted 14 January 2013

Academic Editor: Kota V. Ramana

Copyright © 2013 Celestyna Mila-Kierzenkowska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study was to determine the concentration of lipid peroxidation products, the activity of selected antioxidant and lysosomal enzymes, and protease inhibitor in patients with renal cell carcinoma who underwent radical nephrectomy. The studied group included 44 patients: 21 of them underwent open surgery, while 23 underwent laparoscopy. Blood samples were collected three times: before treatment and 12 hours and five days after nephrectomy. In blood of participants, the concentration of thiobarbituric acid reactive substances (TBARS), the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx), and the activity of acid phosphatase (AcP), arylsulfatase (ASA), cathepsin D (CTSD), and α1-antitrypsin (AAT) were assayed. No statistically significant differences in investigated parameters were found between studied groups. Moreover, TBARS concentration and CAT, SOD, and GPx activity were not altered in the course of both types of surgery. Five days after both open and laparoscopic nephrectomy techniques, AAT activity was higher than its activity 12 hours after the procedure. The obtained results suggest that laparoscopy may be used for nephrectomy as effectively as open surgery without creating greater oxidative stress. Reduced period of convalescence at patients treated with laparoscopy may be due to less severe response of acute-phase proteins.