The Beneficial Effect of Melatonin in Brain Endothelial Cells against Oxygen-Glucose Deprivation Followed by Reperfusion-Induced Injury
Figure 4
The measurement of VEGF expression in bEND.3 cells after OGD/R-induced injury. (a) The level of VEGF was evaluated by immunocytochemistry. This image shows that the expression of VEGF in the experimental control (EC) group was increased compared to the normal control (NC) group. Melatonin attenuated the OGD/R-induced increase in the number of VEGF-positive cells. In Mel (10 nM) and Mel (100 nM) groups, the expression of VEGF was lower than in the EC group. VEGF expression was attenuated in the melatonin treatment group under OGD/R-induced injury. Scale bar: 400 µm. (b) Scale bar: 200 µm, vascular endothelial growth factor (VEGF): green, red, and 4′,6-diamidino-2-phenylindole (DAPI): blue. (c) Western blotting showed that the protein level of VEGF was evidently increased in EC compared to the NC group. The protein level of VEGF was attenuated in both Mel (10 nM) and Mel (100 nM) groups, compared to the EC group. The bar graph shows the quantification of VEGF protein in all groups. β-Actin was used as an internal control. Data are expressed as mean ± S.E.M. (* and **).
