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Oxidative Medicine and Cellular Longevity
Volume 2014, Article ID 706269, 9 pages
Research Article

On the Molecular Pharmacology of Resveratrol on Oxidative Burst Inhibition in Professional Phagocytes

1Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dúbravská 9, 841 04 Bratislava, Slovakia
2Institute of Biophysics AS CR v.v.i. Královopolská 135, 612 65 Brno, Czech Republic
3Institute of Chemical Technology, Faculty of Food and Biochemical Technology, Technická 5, 166 28 Praha 6, Czech Republic
4Institute of Organic Chemistry and Biochemistry AS CR v.v.i., Flemmingovo nám. 2, 166 10 Praha 6, Czech Republic

Received 30 July 2013; Revised 12 December 2013; Accepted 17 December 2013; Published 28 January 2014

Academic Editor: Cristina Angeloni

Copyright © 2014 Radomír Nosáľ et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Resveratrol—3,5,4′-trihydroxystilbene—possesses antioxidant activities in vitro. It dose-dependently inhibited the generation of peroxyl, hydroxyl, peroxides, and lipid peroxidation products in cell free systems. Oxidative burst of whole human blood stimulated with PMA, fMLP, OpZ, and A23187 was inhibited in a concentration-dependent way, indicating suppression of both receptor and nonreceptor activated chemiluminescence by resveratrol. Results from isolated human neutrophils revealed that resveratrol was active extracellularly as well as intracellularly in inhibiting the generation of reactive oxygen species. Liberation of ATP and analysis of apoptosis showed that in the concentration of 100 μM, resveratrol did not change the viability and integrity of isolated neutrophils. Western blot analysis documented that resveratrol in concentrations of 10 and 100 μM significantly decreased PMA-induced phosphorylation of PKC α/βII. Dose-dependent inhibition of nitrite production and iNOS protein expression in RAW 264.7 cells indicated possible interference of resveratrol with reactive nitrogen radical generation in professional phagocytes. The results suggest that resveratrol represents an effective naturally occurring substance with potent pharmacological effect on oxidative burst of human neutrophils and nitric oxide production by macrophages. It should be further investigated for its pharmacological activity against oxidative stress in ischaemia reperfusion, inflammation, and other pathological conditions, particularly neoplasia.