Nrf2 Deficiency Exaggerates Doxorubicin-Induced Cardiotoxicity and Cardiac Dysfunction

Figure 4

Dox-induced activation of Nrf2 in the heart. Male 10–12 wk old WT mice (C57BL/6J) were treated with Dox as in Figure 1 and the hearts (𝑛=4 for each group at indicated time point) were harvested for Q-PCR analysis, Western blot analysis, and immunochemical staining. (a) Left upper panel: Q-PCR analysis of Nrf2 mRNA expression. 𝑛=4, ^∗𝑃<0.05 versus Veh in the same experimental group. Right upper panel: Western blot analysis of Nrf2 protein expression in the heart from mice 4 days after treatment. Lower panel: immunochemical staining of Nrf2 protein in a heart of mouse 4 days after treatment. ^∗𝑃<0.05 versus Veh. (b) Q-PCR analysis of NQO1 and HO-1 mRNA expression. 𝑛=4, ^∗𝑃<0.05 versus Veh in the same experimental group.

(a)

(b)