Apigenin Attenuates Atherogenesis through Inducing Macrophage Apoptosis via Inhibition of AKT Ser473 Phosphorylation and Downregulation of Plasminogen Activator Inhibitor-2

Table 1

MALDI-TOF MS identification of protein molecules with altered expression in OxLDL treated MPM compared with OxLDL + apigenin treated MPM.

Spot number^a	Accession number	Protein score (threshold)^b	Molecular weight^c	Calculated pI value^c	Name of protein	Expression level^d
1	Q8CAQ8 (IMMT_MOUSE)	38 (27)	84247	6.18	Mitochondrial inner membrane protein	↓
2	P12265 (BGLR_MOUSE)	55 (28)	74648	6.22	Beta-glucuronidase	↓
3	Q61107 (GBP4_MOUSE)	74 (25)	71270	6.23	Guanylate-binding protein 4	↓
4	P12265 (BGLR_MOUSE)	143 (28)	74648	6.22	Beta-glucuronidase	↓
5	Q91ZA3 (PCCA_MOUSE)	80 (27)	80498	6.83	Propionyl-CoA carboxylase alpha chain, mitochondrial	↓
6	P58252 (EF2_MOUSE)	264 (27)	96222	6.41	Elongation factor 2	↓
7	N/A^e					↓
8	P60710 (ACTB_MOUSE)	79 (26)	42052	5.29	Actin, cytoplasmic 1	↓
9	P60710 (ACTB_MOUSE)	107 (26)	42052	5.29	Actin, cytoplasmic 1	↓
10	Q9DB05 (SNAA_MOUSE)	106 (26)	33624	5.30	Alpha-soluble NSF attachment protein	↓
11	P12388 (PAI2_MOUSE)	187 (26)	46376	5.04	Plasminogen activator inhibitor 2, macrophage	↑, ⇓
12	P51863 (VA0D1_MOUSE)	198 (27)	40731	4.89	V-type proton ATPase subunit d 1	↓, ⇑
13	P61979 (HNRPK_MOUSE)	47 (27)	51230	5.39	Heterogeneous nuclear ribonucleoprotein K	↓, ⇑
14	P48036 (ANXA5_MOUSE)	192 (28)	35787	4.82	Annexin A5	↓, ⇑
15	P17918 (PCNA_MOUSE)	133 (28)	29108	4.66	Proliferating cell nuclear antigen	↓, ⇑
16	P29351 (PTN6_MOUSE)	134 (28)	67859	7.66	Tyrosine-protein phosphatase nonreceptor type 6	↑, ⇓
17	Q61176 (ARGI1_MOUSE)	37 (27)	34957	6.52	Arginase-1	↑, ⇓
18	P46471 (PRS7_MOUSE)	84 (27)	49016	5.72	26S protease regulatory subunit 7	↑
19	P07724 (ALBU_MOUSE)	102 (26)	70700	5.75	Serum albumin	↑, ⇓
20	Q99K51 (PLST_MOUSE)	138 (27)	71210	5.42	Plastin-3	↑
21	Q8JZX4 (SPF45_MOUSE)	42 (26)	45504	5.65	Splicing factor 45	↑
22	Q60854 (SPB6_MOUSE)	138 (27)	42913	5.53	Serpin B6	⇑
23	Q9CZ13 (QCR1_MOUSE)	122 (27)	53420	5.81	Cytochrome b-c1 complex subunit 1, mitochondrial	↑, ⇓
24	P17182 (ENOA_MOUSE)	189 (26)	47453	6.37	Alpha-enolase	↑, ⇓
25	Q9D154 (ILEUA_MOUSE)	171 (27)	42719	5.85	Leukocyte elastase inhibitor A	↑, ⇓
26	Q9CQ60 (6PGL_MOUSE)	226 (26)	27465	5.55	6-phosphogluconolactonase	↑
27	P60710 (ACTB_MOUSE)	304 (26)	42052	5.29	Actin, cytoplasmic 1	↑
28	P11352 (GPX1_MOUSE)	75 (26)	22544	6.74	Glutathione peroxidase 1	↑, ⇓
29	P09528 (FRIH_MOUSE)	121 (28)	21224	5.53	Ferritin heavy chain	↑

Notes: ^adefined according to spot positions in 2-DE gel indicated, as in Figure 3(a).
^bProtein scores are derived from ions scores as a nonprobabilistic basis for ranking protein hits. Protein scores greater than threshold value are significant ().
^cMolecular weight value calculated by amino acid count; pI value calculated from the database entry without any processing.
^d↑↓ Expression of OxLDL group compared to that of vehicle group; ⇑⇓ expression of apigenin group compared to that of OxLDL group.
^eNo effective peaks of peptide mass fingerprinting were available in spot 7.