Oxidative Medicine and Cellular Longevity / 2015 / Article / Fig 3

Research Article

Glutathione Suppresses Cerebral Infarct Volume and Cell Death after Ischemic Injury: Involvement of FOXO3 Inactivation and Bcl2 Expression

Figure 3

Immunochemical image for confirmation of preserved claudin-5 expression by GSH treatment. (a) Immunochemical images showed that claudin-5-positive cells (green) were particularly reduced in rat cortex of experimental control (EC) group. In GSH treatment group, claudin-5 protein expression was observed in rat cortex compared to the EC group. (b) Ischemic striatum (EC group) showed reduced claudin-5 expression while in GSH treatment group, claudin-5-positive cells were retained in rat striatum owing to GSH treatment. (c) The graph showed the relative intensity percentage (%) of claudin-5 expression in the cortex region to compare the normal control group (as considering that the normal control group’s intensity is 100%). (d) The graph showed the relative intensity percentage (%) of claudin-5 expression in the striatum region to compare the normal control group. Each experiment included 5 repeats per animal. The statistical significance of group differences was determined by one-way analysis of variance (ANOVA) followed by Bonferroni post hoc multiple comparison tests. Differences were considered significant at , . Scale bar = 100 μm, claudin-5: green, 4′, 6-diamidino-2-phenylindole (DAPI): blue. NC: normal control group, EC: experimental control, reperfusion 24 hr after MCAO injury group, and GSH: GSH treatment and reperfusion 24 hr after MCAO injury group.
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