Glutathione Suppresses Cerebral Infarct Volume and Cell Death after Ischemic Injury: Involvement of FOXO3 Inactivation and Bcl2 Expression

Figure 4

Immunocytochemistry to measure ROS generation in bEND.3 cells after H/R-induced injury. bEND.3 cells were treated with GSH for 3 hr before H/R injury. ROS levels were measured using DCF-DA. ROS levels in GSH treatment group were almost the same with the NC (normal control) group. ROS levels in bEND.3 cells were increased in H/R injury exposed group. Under H/R injury, ROS levels in the GSH pretreatment group were decreased compared to H/R injury exposed group. GSH attenuated the H/R-induced increase in DCF-DA-positive cells (green). Each experiment included 4 repeats per condition. 2′, 7′-Dichlorodihydrofluorescein diacetate (DCF-DA): green, scale bar = 600 μm. H/R: hypoxia 6 hr and reperfusion 18 hr injury group.