Glutathione Suppresses Cerebral Infarct Volume and Cell Death after Ischemic Injury: Involvement of FOXO3 Inactivation and Bcl2 Expression

Figure 5

The measurement of PI3K, Akt expression in brain enthothelial cells after H/R-induced injury. (a) Western blotting showed that the relative protein level of PI3K was reduced in EC compared to the NC group. The protein level of PI3K was increased in 1 mM GSH groups, compared to the EC group. The bar graph shows the quantification of PI3K protein in all groups. (b) Western blotting showed that the relative protein level of phosphor-Akt was reduced in EC compared to the NC group. The protein level of phosphor-Akt was increased in 1 mM GSH groups, compared to the EC group. The bar graph shows the quantification of phosphor-Akt/Akt protein in all groups. β-actin was used as an internal control. Each experiment included 3 repeats per condition. Data are expressed as mean ± S.E.M. (, ). Phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and phosphorylated Akt (p-Akt).

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