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Oxidative Medicine and Cellular Longevity
Volume 2015, Article ID 502105, 8 pages
http://dx.doi.org/10.1155/2015/502105
Research Article

Reactive Oxygen Species Are Required for Human Mesenchymal Stem Cells to Initiate Proliferation after the Quiescence Exit

1Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia
2Department of Medical Physics, Institute of Physics, Nanotechnology and Telecommunications, Saint Petersburg State Polytechnic University, Saint Petersburg 195251, Russia
3Federal North-West Medical Research Centre, Saint Petersburg 197341, Russia

Received 8 October 2014; Revised 10 February 2015; Accepted 18 February 2015

Academic Editor: Cristina Angeloni

Copyright © 2015 O. G. Lyublinskaya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study focuses on the involvement of reactive oxygen species (ROS) in the process of mesenchymal stem cells “waking up” and entering the cell cycle after the quiescence. Using human endometrial mesenchymal stem cells (eMSCs), we showed that intracellular basal ROS level is positively correlated with the proliferative status of the cell cultures. Our experiments with the eMSCs synchronized in the G0 phase of the cell cycle revealed a transient increase in the ROS level upon the quiescence exit after stimulation of the cell proliferation. This increase was registered before the eMSC entry to the S-phase of the cell cycle, and elimination of this increase by antioxidants (N-acetyl-L-cysteine, Tempol, and Resveratrol) blocked G1–S-phase transition. Similarly, a cell cycle arrest which resulted from the antioxidant treatment was observed in the experiments with synchronized human mesenchymal stem cells derived from the adipose tissue. Thus, we showed that physiologically relevant level of ROS is required for the initiation of human mesenchymal stem cell proliferation and that low levels of ROS due to the antioxidant treatment can block the stem cell self-renewal.