Nrf2 Signaling and the Slowed Aging Phenotype: Evidence from Long-Lived Models
Nrf2 regulated protein expression in skeletal muscle from Snell dwarf mice. The Pit1 mutation does not influence Nrf2 or Nrf2 target protein expression in skeletal muscle. Nrf2, NQO1, HO-1, SOD-1, and Prdx1 were analyzed by western blotting and normalized to tubulin, shown below the proteins from each blot. Densitometric analyses were conducted on the multiple band of Nrf2. Data are expressed as a ratio of target protein to tubulin (mean ± SEM). males and 10 females of each genotype. Snell dwarf (dw/dw) and heterozygote (dw/+) control mice were bred as the progeny of (DW/J x C3H/HeJ) F1 dw/+ females and (DW/J x C3H/HeJ) F1 dw/dw males .