Tanshinol Rescues the Impaired Bone Formation Elicited by Glucocorticoid Involved in KLF15 Pathway

Figure 2

Tanshinol reverses the imbalance between bone formation and bone resorption. Procedures of treatment in rats were carried out as described in Figure 1, and determinations were executed as follows. (a) Fluorescent micrographs of dual calcein labeling in tibia of rats. (b) Histomorphometric quantitative analysis of dynamic parameters of %L.Pm, MAR, and BFR/TV used as key indicators of bone-forming capacity in tibia spongiosa of rats. (c) Biomarkers of the bone formation including serum BAP, serum OCN, and Col1α1 mRNA level were measured using ELISA assay and qRT-PCR, respectively. (d) Ratio of serum OPG/RANKL reflecting changes of bone turnover and the biomarkers related to bone resorption including serum TRAP5b and OSCAR mRNA level of bone tissue. Data are given as mean ± SD (). versus normal control (Con); versus GC treatment (GC).

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