Tanshinol Rescues the Impaired Bone Formation Elicited by Glucocorticoid Involved in KLF15 Pathway

Figure 3

Tanshinol protects osteoblastic differentiation against GC involved in Wnt signaling and KLF15 transcriptional factor. Rats were treated as in Figure 1, and measurements were made as follows. (a) mRNA levels of Runx2 gene and Osterix gene which contribute to osteoblast differentiation and of Axin2 gene (an indicator of Wnt pathway) were determined by qRT-PCR assay in long bone of rats. (b) mRNA levels of KLF15 gene were detected by qRT-PCR assay in long bone of rats. (c) Expression of β-catenin protein (a key molecule of canonical Wnt signaling) in the left tibia was measured by Western blot method. Representative figure was shown on the left panel, and quantification is shown on the right panel. Data are given as mean ± SD (). versus normal control (Con); versus GC treatment (GC).

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