Tanshinol Rescues the Impaired Bone Formation Elicited by Glucocorticoid Involved in KLF15 Pathway

Figure 4

Tanshinol inhibits oxidative stress mediated by and hampers cellular apoptosis in response to GC. Rats were treated as in Figure 1, and determinations were made as follows. (a) GSR (a critical endogenous antioxidant) activity in bone marrow cells flushed from tibia was assayed using colorimetric assay (). (b) Oxidative stress indicated as amount of ROS generation in bone marrow cells was estimated by DCFH-DA assay, and the fluorescence intensity of DCF was quantified using Image Station 2000 MM assay (). (c) Phosphorylated (a significant mediator of amplification of oxidative stress) in lysates from vertebrae was detected by Western blot assay (). Representative figure was shown on the upper panel, and quantification is shown on the lower panel. (d) Apoptosis in sections was measured by TUNEL staining using fluorescent microscope (). Data are given as mean ± SD. versus normal control (Con); versus GC treatment (GC).

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