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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 1527873, 12 pages
http://dx.doi.org/10.1155/2016/1527873
Research Article

Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes

1Department of Pathology, Instituto Nacional de Cardiología “Ignacio Chavez”, Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico
2Department of Physiology, Instituto Nacional de Cardiología “Ignacio Chavez”, Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico
3Department of Molecular Genetic, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacán, 04510 México City, Mexico
4Department of Pharmacology, Instituto Nacional de Cardiología “Ignacio Chavez”, Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico

Received 17 May 2016; Accepted 25 July 2016

Academic Editor: Mahmoud Balbaa

Copyright © 2016 Israel Pérez-Torres et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2 exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2 deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.