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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 1746985, 7 pages
Research Article

Validation of a Reversed-Phase High Performance Liquid Chromatography Method for the Simultaneous Analysis of Cysteine and Reduced Glutathione in Mouse Organs

1Department of Biomolecular Sciences, University of Urbino “Carlo Bo”, 61029 Urbino, Italy
2Department of Public Health and Infectious Diseases, Pasteur Institute, Cenci-Bolognetti Foundation, “Sapienza” University of Rome, 00161 Rome, Italy
3San Raffaele Pisana Scientific Institute for Research, Hospitalization, and Health Care, 00163 Rome, Italy

Received 4 August 2015; Revised 5 October 2015; Accepted 7 October 2015

Academic Editor: Jara Perez-Jimenez

Copyright © 2016 Serena Brundu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The validated method was applied to determine the concentrations of Cys and GSH in liver, kidney, lungs and heart of C57BL/6 mice. Organs were processed as described in Section 2. Then GSH and Cys were quantified through RP-HPLC method as described in Section 2.

  1. Supplementary Material