Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol

Figure 2

Resveratrol induced gene expressions of multiple antioxidant genes under normal and oxidative conditions. (a) MAC-T cells were treated with resveratrol (50 µM) alone (normal condition) or pretreated with or without resveratrol (50 µM) for 2 h and then treated with 500 μM H₂O₂ (oxidative condition) for the indicated time periods. mRNA expression of HO-1, xCT, Txnrd, and NQO-1 genes was analyzed by real-time PCR. Data are represented as mean ± SD from three experiments. Individual treatments were compared using two-tailed Student’s t-test: (resveratrol group was compared with H₂O₂ control group at specified time points); (resveratrol + H₂O₂ group was compared with H₂O₂ control group at specified time points); (b) MAC-T cells were pretreated with different doses (0–50 µM) of resveratrol for 2 h and then treated with or without H₂O₂ (500 μM) for 8 h. Gene expression was measured by real-time PCR. Data are shown as mean ± SD from three independent experiments. Data were analyzed by one-way ANOVA with the Student-Newman-Keuls method. The means with different superscripts are significantly different ( < 0.05).

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